Identification of constituents of human neutrophil azurophil granules that mediate fungistasis against Histoplasma capsulatum.

Human Neutrophil - mediated Fungistasis against Histoplasma capsulatum

Human neutrophils (PMN) demonstrated potent fungistatic activity against Histoplasma capsulatum (Hc) yeasts in a sensitive microassay that quantifies the growth of yeasts by the incorporation of 13Hjleucine. At a PMN:yeast ratio of 1:2, PMN inhibited the growth of yeasts by 37%. Maximum inhibition of85% to 95% was achieved at a PMN/yeast ratio of 10:1 to 50:1. Opsonization of the yeasts in fres...

Histoplasma capsulatum Fungistatic/Fungicidal Activity against Phagosome Acidification to Mediate Human Macrophages Do Not Require

Biochemical and Morphological Characterization of Azurophil and Specific Granules of Human Neutrophilic Polymorphonuclear Leukocytes

Postnuclear supernates from homogenates of purified neutrophil polymorphonuclear leukocytes (PMNs) from human blood were fractionated by zonal sedimentation and isopycnic equilibration in sucrose gradients. The fractions were characterized biochemically by measuring protein content and the activities of eight enzymes. Selected fractions were further analyzed by electron microscopy. In both cent...

Membrane Proteins in Human Neutrophils Identification and characterization of lipid rafts in subcellular organelles

The human neutrophil is an important effector cell in acute inflammation and in the innate immune response against bacteria and fungi. When immune reactions occur in the tissue in response to antigen challenge, neutrophils are the first cells to enter the site of inflammation. The neutrophil is equipped with a vast amount of receptors that both interact with inflammatory mediators and host tiss...

In situ localization by double-labeling immunoelectron microscopy of anti-neutrophil cytoplasmic autoantibodies in neutrophils and monocytes.

Anti-neutrophil cytoplasmic autoantibodies (ANCA) associated with active Wegener's granulomatosis are directed against a soluble 29-Kd protein present in human neutrophils and monocytes. Affinity labeling with tritiated diisopropylfluorophosphate (3H-DFP) suggested that ANCA-antigen is a serine protease. We used immunoelectron microscopy to study the in situ localization of the ANCA-antigen in ...